Single-Nucleus versus Single-Cell RNA Sequencing of Adult Mouse Kidney

Persistent_ID

https://doi.org/10.25548/14-4KG6

Principal_Investigator

Benjamin Humphreys(Washington University, St. Louis)

Release_Date

2018-11-09

Description

Using adult mouse kidney, we compared single-cell RNA sequencing (scRNA-seq) data generated using the DropSeq platform with single-nucleus RNA sequencing (snRNA-seq) data generated using sNuc-DropSeq, DroNc-seq, and Chromium platforms. We validated snRNA-seq on fibrotic kidney from mice 14 days after unilateral ureteral obstruction (UUO) surgery.

Notice

This page is the corresponding collection tomestone page generated as part of the ATLAS-D2K shutdown. Many links on this page may be broken.

Details

This collection is included in the RBK 2022 paper titled “KIDNEY REPAIR AND REGENERATION: PERSPECTIVES OF THE (RE)BUILDING A KIDNEY CONSORTIUM”. The figure below is included as “Figure 4: Leveraging the data hub to reveal intercellular communication networks between Proximal Tubular Cells, Fibroblasts, and Macrophages” in the paper.

The data reveals intercellular communication networks.

• A) Annotated clusters from a unilateral ureteral obstruction kidney analyzed by snRNA-seq.
• B,C) The chemokine Ccl2 is excusively expressed in dedifferentiated proximal tubule, and are known to upregulate Ccl2.
• D, E) The Ccl2 receptors Ccr2 and Ccr4 are expressed in macrophages and activated fibroblasts, respectively, suggesting that dedifferentiated proximal tubule signals via Ccr2 to these two interstitial cell types. {height=400}

Consortium

(Re)Building a Kidney (RBK) Consortium