Protocols Library

• A Commercially Available Kit to Generate Human Kidney Organoids (Version 1.0)
• Adult Mouse DRG Dissociation for FACS of TrpV1PLAP nLacZ Mice (Version 1.0)
• Adult Mouse Kidney Dissociation (Version 1.0)
• Adult mouse DRG dissociation for FACS, 060214 (Version 1.0)
• Adult mouse kidney dissociation (on ice) (Version 1.0)
• Annotation Guidelines for Wholemount In Situ Hybridization (Version 1.0)
• Antibody Staining Protocol on Frozen Kidney Sections (Version 1.0)
• Assay for cell pH (pHi) and basolateral Na+/H+ exchange in nonperfused tubules using BCECF (Version 1.0)
• Assay for measurement of intracellular calcium concentration [Ca2+]i in nonperfused isolated tubules or structures using the ratiometric indicator FURA-2 AM (Version 1.0)
• Assay for measurement of intracellular sodium concentration ([Na+]i) and basolateral Na+/K+ ATPase activity in nonperfused isolated tubules or structures using the fluorescent indicator CoroNa Green. (Version 1.0)
• Assembling 3D Devices With Photos (Version 1.0)
• Assessing Ion Channel Expression by Patch Clamp (Version 1.0)
• Bulk RNA extraction from low numbers of FACS-sorted cells (Version 2.0)
• Cell seeding protocol for 3D silk scaffolds (Version 1.0)
• Cryostat Sectioning (Version 1.0)
• Determination of Cellular Localization of ISH Patterns (Version 1.0)
• Dextran Accumulation in Human Kidney Organoids (Version 1.0)
• Digestion protocol for kidney single cell suspension (Version 1.0)
• Digoxigenin-11-UTP-Labeled Riboprobe Synthesis (Version 1.0)
• Digoxigenin-Labeled In Situ Hybridization for E15.5 Kidneys (Version 1.0)
• Digoxigenin-Labeled In Situ Hybridization for P1 Mouse Kidney Sections (Version 1.0)
• Digoxigenin-labeled in-situ hybridization of urogenital sections (Version 1.0)
• Digoxigenin-labeled riboprobe synthesis from plasmid template (Version 1.0)
• Digoxigenin-labelled Riboprobe Synthesis from a PCR-generated DNA template (Version 1.0)
• Digoxygenin-labeled in situ Hybridization protocol on Frozen Kidney Sections (Version 1.0)
• Dissociating adult human kidney tissue (on ice) (Version 1.0)
• E12.5 & E14.5 mouse DRG dissociation for FACS, 053114 (Version 1.0)
• E18.5 mouse DRG dissociation for FACS, 060214 (Version 1.0)
• EGFP Labeled Immortalized Podocyte Culture (Version 1.0)
• Embedding 3D MPS Devices for Sectioning (Version 1.0)
• Embryonic Kidney Dissociation to single cells (Version 1.0)
• FACS Protocol (Version 1.0)
• FACS analysis for E15.5 mouse kidneys (Version 1.0)
• Fluorescent ISH – Freedom Evo (Version 1.0)
• Fluorescent Immunocytochemistry Protocol on Frozen sections (Version 1.0)
• Fluorescent Immunohistochemistry on Embryo Cryosections (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Annotation Tool checking protocols (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Automated checking protocols - Image Checker (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Automated checking protocols - Probe Checker (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Automated checking protocols - Spreadsheet Checker (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Data Submission Flow (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Genelists from Microarray Analysis (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Manual checking protocols - ISH / IHC / Tg Data (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Manual checking protocols - Microarray Data (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Normalisation of cDNA microarray CHP files (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Quality Control - ISH (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: SOFT mapping protocol (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Software Testing and Bug Fixing (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Standards for GUDMAP in situ hybridisation and immunohistochemistry entries (Version 1.0)
• GUDMAP1/GUDMAP2/nGUDMAP: Standards for GUDMAP microarray entries (Version 1.0)
• Generating kidney organoids from human pluripotent stem cells. (Version 2.0)
• Generation of kidney organoids from human pluripotent stem cells (Version 1.0)
• Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells (Version 1.0)
• Human induced Pluripotent Stem Cell (HiPSC) culture protocol for maintenance, passaging and cryostorage (Version 1.0)
• Hyb Solution Guide (Version 1.0)
• Imaging isolated and in situ bladder arterioles (Version 1.0)
• Immortalized Mouse Endothelial Cell Culture (Version 1.0)
• Immunofluorescent analysis of antibodies on kidney sections (Version 1.0)
• Immunohistochemistry (IHC) for In-situ Hybridization (ISH)-stained Mouse Sections (Version 1.0)
• Immunohistochemistry - Paraffin section (Version 1.0)
• Immunohistochemistry on paraffin sections (Version 1.0)
• Immunohistochemistry on sections of organs and pelvic ganglia (Version 1.0)
• Immunostaining FFPE Human Kidney Tissue (Version 1.0)
• In Situ Hybridization – Freedom Evo (Version 1.0)
• In-situ Hybridization on Vibrating Microtome-cut Mouse Tissue Sections (Version 1.0)
• Isolation and culture of CITED1+ nephron progenitor cells (Version 1.0)
• Isolation of transgene-expressing (e.g. GFP+) sensory neuron progenitors from fetal and postnatal mouse DRG by flow sorting (Version 1.0)
• Isolation of tubule fragments from organoids (Version 1.0)
• Isolation, propogation and differentiation of mouse Nephrogenic Zone Cells (NZCs) (Version 1.0)
• Kim1-GCE;tdTom Cell isolation protocol (Version 1.0)
• LCM RNA preparation (Version 1.0)
• Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells (Version 1.0)
• Laser Capture Microdissection (Adapted from the Arcturus protocols) (Version 1.0)
• Laser Capture Microdissection (Version 1.0)
• Library construction protocol for nanogram amounts of total RNA for RNAseq - LOW VOLUME (Version 1.0)
• Library construction protocol for nanogram amounts of total RNA for RNAseq – HIGH VOLUME (Version 1.0)
• Mapping TRPV1-lineage in arteriolar smooth muscle of bladder (Version 1.0)
• Measuring transepithelial resistance and voltage using human pluripotent stem cell-derived novel collecting duct principal and intercalated cells (Version 1.0)
• Method for analyzing RNA following intracellular sorting (MARIS) Of nephron progenitors (Version 1.0)
• Microarray Screening (Version 1.0)
• Microarrays (Version 1.0)
• Multiplex Immunofluorescence on Formalin-Fixed Paraffin-Embedded Mouse Tissue (Version 1.0)
• One-color FISH (Version 1.0)
• Optical Projection Tomography (OPT) (Version 1.0)
• Optimized Vibratome Section in situ Hybridization For P1 Mouse Kidney Sections (Version 1.0)
• Organic anion and cation transport assay for kidney organoids (Version 1.0)
• Organoids from asynchronously mixed directed differentiation cells (Version 1.0)
• PCR-based Digoxigenin-labeled RNA probe synthesis and in situ hybridization on paraffin-embedded E15.5 murine kidney (Version 1.0)
• Placental alkaline phosphatase (PLAP) staining of bladder sections (Version 1.0)
• Placental alkaline phosphatase (PLAP) staining of bladder whole mounts (Version 1.0)
• Podocyte Differentiation from Nephron Progenitor Cells (Version 1.0)
• Preparing OCT blocks of kidney tissue (Version 1.0)
• Processing slides for LCM (Version 1.0)
• Protocol for kidney regeneration and new nephron development in adult zebrafish by gentamicin induced injury (Version 1.0)
• Psychrophilic (Cold) protease dissociation of mouse kidney (Version 1.0)
• QC of RNAseq data from cell/tissue populations (not single cells) (Version 1.0)
• Quenching Auto-fluorescence with Cupric Sulfate Buffer (Version 1.0)
• RNA Amplification (Version 1.0)
• RNA Isolation (Version 1.0)
• RNA Isolation Procedure from Flow Sorted Cells (Version 1.0)
• RNA amplification and labeling of LCM samples for microarrays (Version 1.0)
• RNA extraction and sample preparation for Affymetrix Gene 1.0 ST arrays (Version 1.0)
• RNA isolation from LCM samples (Version 1.0)
• RNA isolation from whole testis (Version 1.0)
• Rat Tail Collagen Extraction (Version 1.0)
• Real-time drug efflux in human kidney organoid (Version 1.0)
• Real-time organic cation transport in human kidney organoid (Version 1.0)
• Riboprobe Synthesis (Version 1.0)
• Riboprobe Synthesis for In Situ Hybridization (Version 1.0)
• Riboprobe synthesis from a plasmid template (Version 1.0)
• Section in situ hybridization (SISH) on kidney sections (Version 1.0)
• Sectioning (Version 1.0)
• Sectioning of Paraformaldehyde-fixed Mouse Urogenital Sinus (UGS) Tissues with a Vibrating Microtome (Version 1.0)
• Semi-Automated Section in situ Hybridisation for 7µm paraffin sections (Version 1.0)
• Semi-Automated Whole Mount in situ Hybridisation (Version 1.0)
• Sex genotyping of mice (Version 1.0)
• Simultaneous reprogramming and gene editing of human fibroblasts (Version 1.0)
• Staining Protocol 3D MPS Devices (Version 1.0)
• Staining of frozen sections for LCM (Version 1.0)
• Target Amplification (Version 1.0)
• Tissue Collection Protocol for Laser Capture Micro-dissection (LCM) of 13.5dpc Mouse Urethra and Hindgut (Version 1.0)
• Tissue isolation and embedding in OCT (Optimal Cutting Temperature Compound) (Version 1.0)
• Two-color FISH (Version 1.0)
• Whole mount imaging of TRPV1-lineage expression in mouse bladder (Version 1.0)
• Whole mount immunofluorescent analysis of human cortical kidney slices (Version 1.0)
• Whole mount in situ hybridization (Version 1.0)
• Whole-Mount Indirect Fluorescent Immunohistochemistry – Double or multiple staining (Version 1.0)
• Wholemount immunofluorescence staining, tissue clearing, and 3-D imaging (Version 1.0)
• qPCR assay to evaluate nephron repair/failed repair using MACS-purification of tubular cells from cisplatin-injured organoids (Version 1.0)