Tissue isolation and embedding in OCT (Optimal Cutting Temperature Compound) (Version 1.0)

Version

1.0

Notice

This page is the corresponding protocol tomestone page generated as part of the ATLAS-D2K shutdown in July 2025. Many links on this page may be broken.

Authors

Jim Lessard

Keywords

[‘Laser Capture Microdissection’, ‘histology’]

Subjects

[‘Cell biology’]

Release Date

2017-08-04

Abstract

Tissue isolation and embedding in OCT (Optimal Cutting Temperature Compound) protocol by the Lessard Group

Procedure

1. Dissect tissue (e.g. bladder, ureter, etc.) rapidly and place each specimen in OCT using a plastic mold (Fisher Scientific).
2. Be sure the specimen is covered in central position, not too near the top and in the desired orientation for sectioning.
3. Immediately freeze the sample by placing it on dry ice.
4. When the OCT is completely frozen keep the mold in dry ice, and then store long term in liquid nitrogen freezer or at –80°C.

Consortium

GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Consortium