Digoxigenin-Labeled In Situ Hybridization for E15.5 Kidneys (Version 1.0)

Version

1.0

Notice

This page is the corresponding protocol tomestone page generated as part of the ATLAS-D2K shutdown in July 2025. Many links on this page may be broken.

Authors

Todd Valerius; Jing Yu

Keywords

[‘in situ hybridization’]

Subjects

[‘Gene expression analysis’, ‘Developmental biology’, ‘Molecular biology’]

Release Date

2017-08-04

Abstract

Reagent Testing – In conducting a large scale, continuous screen, it is critical to fully test each reagent to avoid unnecessary disasters and maintain data consistency. New batches of Proteinase K, antibody, and lots of BM purple need to be carefully tracked. Testing is best done with small numbers (6-12) of wildtype UGSs. Making reagents in large tested batches is ideal. The sources of reagents should also be constant as we have had SDS from a different company cause background issues.

Procedure

See PDF.

Consortium

GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Consortium