Riboprobe Synthesis for In Situ Hybridization (Version 1.0)

Version

1.0

Notice

This page is the corresponding protocol tomestone page generated as part of the ATLAS-D2K shutdown in July 2025. Many links on this page may be broken.

Authors

Todd Valerius; Jing Yu

Keywords

[‘in situ hybridization’, ‘probe generation’]

Subjects

[‘Gene expression analysis’, ‘Molecular biology’]

Release Date

2017-08-04

Abstract

Riboprobe Synthesis for In Situ Hybridization protocol by McMahon Group

Procedure

See PDF.

Acknowledgement

Based on Andy McMahon Lab protocol (and references therein), modified by Karl Staser, M. Todd Valerius, and Jing Yu.

Created 10/22/02, modified 2/14/05 and 7/22/05.

The in situ hybridization methods were developed by Dave Wilkinson from protocols from Phil Ingham, Ron Conlon, Barry Rosen, and Richard Harland in David Wilkinson, ed., In Situ Hybridization: A Practical Approach, Oxford: IRL Press, 1992.

Consortium

GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Consortium