Laser Capture Microdissection (Version 1.0)

Version

1.0

Notice

This page is the corresponding protocol tomestone page generated as part of the ATLAS-D2K shutdown in July 2025. Many links on this page may be broken.

Authors

Steve Potter

Keywords

[‘Laser Capture Microdissection’]

Subjects

[‘Isolation, purification and separation’, ‘Gene expression analysis’]

Release Date

2017-08-07

Abstract

Laser Capture Microdissection protocol by the Potter Group

Procedure

1. Use the Veritas machine, (or palm zeiss when available), with membrane slides, pretty much as per standard protocols, but with following considerations.
2. Too strong a cutting laser might degrade RNA according to Chris Erwin. So cut back on power as much as possible. I’ve had good luck with the setting at 3.0.
3. Be careful to avoid cap crap. But if there is a little then can use ablation to remove. But be careful to re-set laser after ablation, so don’t ruin sample.
4. Label caps clearly and take photos before, after and of caps to record material captured.
5. Place caps in 0.5 ul tubes after capture is complete.
6. Store on dry ice, then at –80oC.

Consortium

GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Consortium