Target Amplification (Version 1.0)

Version

1.0

Notice

This page is the corresponding protocol tomestone page generated as part of the ATLAS-D2K shutdown in July 2025. Many links on this page may be broken.

Authors

Steve Potter

Keywords

[‘RNA Expression’]

Subjects

[‘Isolation, purification and separation’, ‘Gene expression analysis’]

Release Date

2017-08-07

Abstract

Target Amplification protocol by the Potter Group

Procedure

Use TargetAmp 2-round aminoallyl amplification kit from Epicentre biotechnologies.

Add an additional 3/4 ul of SBI semi-random primer at step one, before speed-vacing to 3 ul.

Also, add 1 ul of this primer for part F, step one, in addition to the Epicentre primer.

Consortium

GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Consortium