RNA amplification and labeling of LCM samples for microarrays (Version 1.0)

Version

1.0

Notice

This page is the corresponding protocol tomestone page generated as part of the ATLAS-D2K shutdown in July 2025. Many links on this page may be broken.

Authors

Kevin Gaido

Keywords

[‘Laser Capture Microdissection’, ‘RNA preparation’]

Subjects

[‘Isolation, purification and separation’, ‘Gene expression analysis’]

Release Date

2017-08-07

Abstract

RNA amplification and labeling of LCM samples for microarrays protocols by the Gaido Group.

Procedure

Proposed kit and protocol to be followed is Epicentre TargetAmp 2-Round Aminoallyl-aRNA Amplification kit 1.0 (catalogue # TAA2R4924).

Evaluation of this kit is still in progress.

Consortium

GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Consortium